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quantitec reverse transcriptase kit  (Qiagen)


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    Structured Review

    Qiagen quantitec reverse transcriptase kit
    Quantitec Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantitec+reverse+transcriptase+kit/Reverse+transcriptase/pmc12126455-179-10-17
    Average 99 stars, based on 7639 article reviews
    quantitec reverse transcriptase kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Age-dependent electrical and morphological remodeling of the Drosophila heart caused by hERG/ seizure mutations
    Article Snippet: RNA was extracted using QIAzol (QIAGEN, Ref# 79306) and purified using miRNeasy Mini Kit (QIAGEN, Ref# 217004) following manufacturer’s instructions for RNA purification of small biological sample sizes. .. Genomic DNA removal and cDNA synthesis was done using Quantitec Reverse Transcriptase kit (QIAGEN, Ref# 205311; see for primer sequences). .. Quantitative PCRs were carried out with the LightCycler 96 Instrument (Roche, Ref# 05815916001) and FastStart Essential DNA Green Master kit (Roche, Ref# 06402712001) was used for SYBR Green I-based real-time PCR.

    Reverse Transcription:

    Article Title: Age-dependent electrical and morphological remodeling of the Drosophila heart caused by hERG/ seizure mutations
    Article Snippet: RNA was extracted using QIAzol (QIAGEN, Ref# 79306) and purified using miRNeasy Mini Kit (QIAGEN, Ref# 217004) following manufacturer’s instructions for RNA purification of small biological sample sizes. .. Genomic DNA removal and cDNA synthesis was done using Quantitec Reverse Transcriptase kit (QIAGEN, Ref# 205311; see for primer sequences). .. Quantitative PCRs were carried out with the LightCycler 96 Instrument (Roche, Ref# 05815916001) and FastStart Essential DNA Green Master kit (Roche, Ref# 06402712001) was used for SYBR Green I-based real-time PCR.

    Article Title: PHA-4/Foxa is essential and specific for dietary restriction induced longevity in C. elegans
    Article Snippet: .. Total RNA was extracted using TRIzol reagent (GIBCO). cDNA was created using the Quantitec Reverse Transcriptase kit (Qiagen). ..

    Article Title: Munc18-2 deficiency causes familial hemophagocytic lymphohistiocytosis type 5 and impairs cytotoxic granule exocytosis in patient NK cells
    Article Snippet: .. Total RNA was reverse transcribed using a Quantitec Reverse Transcriptase kit (Qiagen). ..

    Article Title: Modulatory effect of metformin and its transporters on immune infiltration in tumor microenvironment: a bioinformatic study with experimental validation.
    Article Snippet: 2.4.2.1 Quantitative real‐time polymerase chain reaction (qRT‐PCR) Total RNA was extracted using TRIzolTM reagent (Catalog number: 15596026, InvitrogenTM—Life Technologies Inc, Thermo Fischer, USA). .. Then, RNA was reverse transcribed into complementary DNA (cDNA) using QuantiTec Reverse Transcriptase Kit (Catalog number: 205311, Qiagen, USA). .. The Maximas SYBR Green/Fluorescein qPCR Master Mix (Catalog number: K0241, InvitrogenTM—Life Technologies Inc, Thermo Fischer, USA) was used to cDNA amplification.

    Article Title: Neuroendocrine Coordination of Mitochondrial Stress Signaling and Proteostasis
    Article Snippet: .. Total mRNA was extracted using freeze cracking with QIAzol reagent and subsequent chloroform treatment, before undergoing ethanol and isopropanol wash steps. mRNA underwent column purification on QIAGEN RNAeasy columns. cDNA was created using the Quantitec Reverse Transcriptase kit according to standard protocol (QIAGEN). .. SybrGreen real-time QPCR experiments were performed as described in the manual using Applied Biosystems QuantStudio 6 Flex and cDNA at a 1:20 dilution.

    Article Title: Modulatory effect of metformin and its transporters on immune infiltration in tumor microenvironment: a bioinformatic study with experimental validation
    Article Snippet: Total RNA was extracted using TRIzolTM reagent (Catalog number: 15596026, InvitrogenTM—Life Technologies Inc, Thermo Fischer, USA). .. Then, RNA was reverse transcribed into complementary DNA (cDNA) using QuantiTec Reverse Transcriptase Kit (Catalog number: 205311, Qiagen, USA). .. The Maximas SYBR Green/Fluorescein qPCR Master Mix (Catalog number: K0241, InvitrogenTM—Life Technologies Inc, Thermo Fischer, USA) was used to cDNA amplification.

    other:

    Article Title: Neuroendocrine Coordination of Mitochondrial Stress Signaling and Proteostasis
    Article Snippet: SybrGreen real-time QPCR experiments were performed as described in the manual using Applied Biosystems QuantStudio 6 Flex and cDNA at a 1:20 dilution.

    Purification:

    Article Title: Neuroendocrine Coordination of Mitochondrial Stress Signaling and Proteostasis
    Article Snippet: .. Total mRNA was extracted using freeze cracking with QIAzol reagent and subsequent chloroform treatment, before undergoing ethanol and isopropanol wash steps. mRNA underwent column purification on QIAGEN RNAeasy columns. cDNA was created using the Quantitec Reverse Transcriptase kit according to standard protocol (QIAGEN). .. SybrGreen real-time QPCR experiments were performed as described in the manual using Applied Biosystems QuantStudio 6 Flex and cDNA at a 1:20 dilution.



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